14.12 Red cell folate (categories)

Red cell folate data available for STOP cohort only.


Within reference ranges (360 to 1400 vs >1400).

14.12.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 310.796656 1 <0.0001 ***
rcfolate_gp 0.792747 1 0.4 ns
GA_samp 92.716282 1 <0.0001 ***
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 33.7346509 1 <0.0001 ***
rcfolate_gp 0.3751633 1 0.5 ns
GA_samp 1668.8334228 1 <0.0001 ***
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 554.223806 1 <0.0001 ***
rcfolate_gp 4.402085 1 0.04 *
GA_samp 774.499623 1 <0.0001 ***
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp 1 Inf 3.454 3.454 0.06 ns
GA_samp 1 Inf 3.104 3.104 0.08 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp 1 Inf 3.124 3.124 0.08 ns
GA_samp 1 Inf 2.451 2.451 0.1 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
rcfolate_gp 1 Inf 2.923 2.923 0.09 ns
GA_samp 1 Inf 0.679 0.679 0.4 ns

14.12.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 18.02 (13.07, 24.84) <0.0001
rcfolate_gp>1400 1.04 (0.96, 1.12) 0.37
GA_samp 1.13 (1.10, 1.16) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.70 (1.42, 2.04) <0.0001
rcfolate_gp>1400 0.99 (0.94, 1.03) 0.54
GA_samp 1.36 (1.34, 1.38) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.06 (0.05, 0.07) <0.0001
rcfolate_gp>1400 0.93 (0.88, 1.00) 0.04
GA_samp 1.33 (1.30, 1.35) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 7.78 (5.31, 11.41) <0.0001
rcfolate_gp>1400 1.10 (0.99, 1.23) 0.06
GA_samp 1.03 (1.00, 1.07) 0.08
HOMA-IR
Var Effect P
(Intercept) 1.68 (1.12, 2.51) 0.01
rcfolate_gp>1400 1.10 (0.99, 1.23) 0.08
GA_samp 1.03 (0.99, 1.07) 0.12
Glucose (mmol/L)
Var Effect P
(Intercept) 4.83 (4.51, 5.17) <0.0001
rcfolate_gp>1400 1.02 (1.00, 1.04) 0.09
GA_samp 1.00 (0.99, 1.00) 0.41

14.12.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.12.4 Estimated marginal means

Outcome rcfolate_gp response SE df lower.CL upper.CL
PRL (ng/ml) 360 to 1400 74.124 5.629 72.984 63.712 86.237
PRL (ng/ml) >1400 76.868 5.697 66.388 66.297 89.125
hPL (μg/ml) 360 to 1400 58.879 1.899 74.820 55.214 62.787
hPL (μg/ml) >1400 58.014 1.786 62.513 54.551 61.696
GH2 (ng/ml) 360 to 1400 1.502 0.053 115.968 1.400 1.611
GH2 (ng/ml) >1400 1.404 0.047 89.453 1.315 1.500
Insulin (uU/ml) 360 to 1400 10.962 0.424 Inf 10.163 11.825
Insulin (uU/ml) >1400 12.107 0.445 Inf 11.265 13.012
HOMA-IR 360 to 1400 2.312 0.094 Inf 2.135 2.504
HOMA-IR >1400 2.555 0.099 Inf 2.367 2.757
Glucose (mmol/L) 360 to 1400 4.703 0.042 Inf 4.622 4.785
Glucose (mmol/L) >1400 4.799 0.038 Inf 4.726 4.873

14.12.5 Plots

14.12.6 Post-hoc comparisons

Outcome Contrast Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) >1400 / 360 to 1400 1.04 (0.96, 1.12) 0.4
hPL (μg/ml) >1400 / 360 to 1400 0.99 (0.94, 1.03) 0.5
GH2 (ng/ml) >1400 / 360 to 1400 0.93 (0.88, 1.00) 0.04
Insulin (uU/ml) >1400 / 360 to 1400 1.10 (0.99, 1.23) 0.06
HOMA-IR >1400 / 360 to 1400 1.10 (0.99, 1.23) 0.08
Glucose (mmol/L) >1400 / 360 to 1400 1.02 (1.00, 1.04) 0.09