14.12 Red cell folate (categories)

Red cell folate data available for STOP cohort only.

Within reference ranges (360 to 1400 vs >1400).

14.12.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	310.796656	1	<0.0001	***
rcfolate_gp	0.792747	1	0.4	ns
GA_samp	92.716282	1	<0.0001	***

hPL (μg/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	33.7346509	1	<0.0001	***
rcfolate_gp	0.3751633	1	0.5	ns
GA_samp	1668.8334228	1	<0.0001	***

GH2 (ng/ml)

	Chisq	Df	Pr(>Chisq)
(Intercept)	554.223806	1	<0.0001	***
rcfolate_gp	4.402085	1	0.04	*
GA_samp	774.499623	1	<0.0001	***

Insulin (uU/ml)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp	1	Inf	3.454	3.454	0.06	ns
GA_samp	1	Inf	3.104	3.104	0.08	ns

HOMA-IR

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp	1	Inf	3.124	3.124	0.08	ns
GA_samp	1	Inf	2.451	2.451	0.1	ns

Glucose (mmol/L)

model term	df1	df2	F.ratio	Chisq	p.value
rcfolate_gp	1	Inf	2.923	2.923	0.09	ns
GA_samp	1	Inf	0.679	0.679	0.4	ns

14.12.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)

Var	Effect	P
(Intercept)	18.02 (13.07, 24.84)	<0.0001
rcfolate_gp>1400	1.04 (0.96, 1.12)	0.37
GA_samp	1.13 (1.10, 1.16)	<0.0001

hPL (μg/ml)

Var	Effect	P
(Intercept)	1.70 (1.42, 2.04)	<0.0001
rcfolate_gp>1400	0.99 (0.94, 1.03)	0.54
GA_samp	1.36 (1.34, 1.38)	<0.0001

GH2 (ng/ml)

Var	Effect	P
(Intercept)	0.06 (0.05, 0.07)	<0.0001
rcfolate_gp>1400	0.93 (0.88, 1.00)	0.04
GA_samp	1.33 (1.30, 1.35)	<0.0001

Insulin (uU/ml)

Var	Effect	P
(Intercept)	7.78 (5.31, 11.41)	<0.0001
rcfolate_gp>1400	1.10 (0.99, 1.23)	0.06
GA_samp	1.03 (1.00, 1.07)	0.08

HOMA-IR

Var	Effect	P
(Intercept)	1.68 (1.12, 2.51)	0.01
rcfolate_gp>1400	1.10 (0.99, 1.23)	0.08
GA_samp	1.03 (0.99, 1.07)	0.12

Glucose (mmol/L)

Var	Effect	P
(Intercept)	4.83 (4.51, 5.17)	<0.0001
rcfolate_gp>1400	1.02 (1.00, 1.04)	0.09
GA_samp	1.00 (0.99, 1.00)	0.41

14.12.3 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.12.4 Estimated marginal means

Outcome	rcfolate_gp	response	SE	df	lower.CL	upper.CL
PRL (ng/ml)	360 to 1400	74.124	5.629	72.984	63.712	86.237
PRL (ng/ml)	>1400	76.868	5.697	66.388	66.297	89.125
hPL (μg/ml)	360 to 1400	58.879	1.899	74.820	55.214	62.787
hPL (μg/ml)	>1400	58.014	1.786	62.513	54.551	61.696
GH2 (ng/ml)	360 to 1400	1.502	0.053	115.968	1.400	1.611
GH2 (ng/ml)	>1400	1.404	0.047	89.453	1.315	1.500
Insulin (uU/ml)	360 to 1400	10.962	0.424	Inf	10.163	11.825
Insulin (uU/ml)	>1400	12.107	0.445	Inf	11.265	13.012
HOMA-IR	360 to 1400	2.312	0.094	Inf	2.135	2.504
HOMA-IR	>1400	2.555	0.099	Inf	2.367	2.757
Glucose (mmol/L)	360 to 1400	4.703	0.042	Inf	4.622	4.785
Glucose (mmol/L)	>1400	4.799	0.038	Inf	4.726	4.873

14.12.5 Plots

14.12.6 Post-hoc comparisons

Outcome	Contrast	Ratio of Geometric means (95% CI)	P-value
PRL (ng/ml)	>1400 / 360 to 1400	1.04 (0.96, 1.12)	0.4
hPL (μg/ml)	>1400 / 360 to 1400	0.99 (0.94, 1.03)	0.5
GH2 (ng/ml)	>1400 / 360 to 1400	0.93 (0.88, 1.00)	0.04
Insulin (uU/ml)	>1400 / 360 to 1400	1.10 (0.99, 1.23)	0.06
HOMA-IR	>1400 / 360 to 1400	1.10 (0.99, 1.23)	0.08
Glucose (mmol/L)	>1400 / 360 to 1400	1.02 (1.00, 1.04)	0.09